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2× universal pcr premix buffer  (TransGen biotech co)

 
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    TransGen biotech co 2× universal pcr premix buffer
    2× Universal Pcr Premix Buffer, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2%C3%97+universal+pcr+premix+buffer/2%C3%97+universal+pcr+premix+buffer/pmc11341834-130-23-27
    Average 90 stars, based on 1 article reviews
    2× universal pcr premix buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: UPCARE: Urinary Extracellular Vesicles‐Derived Prostate Cancer Assessment for Risk Evaluation
    Article Snippet: .. In the subsequent one‐step RT‐qPCR reactions, prepare PCR reaction system A for amplifying HOCX6, KLK3 and DLX1 genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 1.44 μL primer mix, 1.23 μL probe mix, 10 μL nucleic acid template, and 2.33 μL DNase and RNase‐free water; Prepare PCR reaction system B for amplifying MALAT1, SPDEF, FOXA1 and PSMA genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 1.92 μL primer mix, 1.74 μL probe mix, 10 μL nucleic acid template, and 1.34 μL DNase and RNase‐free water; Prepare PCR reaction system C for amplifying PCA3, AMACR and ERG genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 0.94 μL primer mix, 1.15 μL probe mix, 10 μL nucleic acid template, and 2.9 μL DNase and RNase‐free water. .. RT‐qPCR was performed using a real‐time PCR system (Applied Biosystems 7500).

    Reverse Transcription:

    Article Title: UPCARE: Urinary Extracellular Vesicles‐Derived Prostate Cancer Assessment for Risk Evaluation
    Article Snippet: .. In the subsequent one‐step RT‐qPCR reactions, prepare PCR reaction system A for amplifying HOCX6, KLK3 and DLX1 genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 1.44 μL primer mix, 1.23 μL probe mix, 10 μL nucleic acid template, and 2.33 μL DNase and RNase‐free water; Prepare PCR reaction system B for amplifying MALAT1, SPDEF, FOXA1 and PSMA genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 1.92 μL primer mix, 1.74 μL probe mix, 10 μL nucleic acid template, and 1.34 μL DNase and RNase‐free water; Prepare PCR reaction system C for amplifying PCA3, AMACR and ERG genes, including 15 μL of 2× universal PCR premix buffer (TransGen Biotech), 1.5 μL reverse transcriptase and DNA polymerase mix, 0.94 μL primer mix, 1.15 μL probe mix, 10 μL nucleic acid template, and 2.9 μL DNase and RNase‐free water. .. RT‐qPCR was performed using a real‐time PCR system (Applied Biosystems 7500).



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    2× universal pcr premix buffer  (TransGen biotech co)
    90
    TransGen biotech co 2× universal pcr premix buffer
    2× Universal Pcr Premix Buffer, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2%C3%97+universal+pcr+premix+buffer/2%C3%97+universal+pcr+premix+buffer/pmc11341834-130-23-27
    Average 90 stars, based on 1 article reviews
    2× universal pcr premix buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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